Fig. 3. PGs of CS and heparin, and proteases did not contribute to outline the [Ca²⁺]_i signal elicited by mast cells mediator cocktail. A) Trace illustrating a control MCM-evoked response. B) The response did not change after incubation with FSLLRY-NH2. C) Neither pre-digestion of MCM with chondroitinase ABC nor D) heparinase modified [Ca²⁺]_i transients. E) [Ca²⁺]_i signal amplitude (peak) and F) area under curve (AUC) are presented as % of means ± SEM.